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KMID : 0385520130260060353
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Analytical Science & Technology
2013 Volume.26 No. 6 p.353 ~ p.356
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Spectrofluorometric determination of caffeine using acridine orange-β-cyclodextrin inclusion complex
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Park Jong-Hee
Choi Hee-Seon
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Abstract
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A method on the determination of caffeine in beverage with acridiene orange-β-cyclodextrin (CD) inclusion complex was developed. The conditions such as pH of the sample solution and concentration of acridine orange and β-CD were optimized to 12.0(±0.5), 1.9 × 10?6 M and 1.25 × 10?3 M, respectively. Under these optimum conditions, the calibration curve of caffeine was obtained over concentration range of 5 × 10?5~ 1.1 × 10?3 M. The detection limit was 1.0 × 10?5 M. The relative errors(%) in beverage samples were less than 5.0%.
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KEYWORD
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caffeine determination, acridine orange, B-cyclodextrin, spectrofluorometry,
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